Chemiluminescence is the process in which light is emitted as a result of a chemical reaction. It differs from fluorescence in the manner that the electrons in an excited state are the result of a chemical reaction and not due to the absorption of a photon. It is an antithesis photochemical reaction, in which the light is used to make an endothermic chemical reaction take place.
In this, the light is produced from an exothermic reaction. Enhanced chemiluminescence is a technique used to do a variety of detection assays in the field of biology. You can buy a high-quality chemiluminescence detection kit at https://www.bosterbio.com/enhanced-chemiluminescent-reagent-kit-mouse-igm-for-western-blotting-ek1005-boster.html.
A horseradish peroxidase enzyme is induced into an antibody that specifically identifies the molecule of interest. This enzyme mixture then catalyzes the conversion of the enhanced chemiluminescent into a sensitized reagent present in the premises of the molecule of interest which emits light at the degradation of the one carbonyl.
Enhanced chemiluminescence technique allows the detection of tiny quantities of a biomolecule. A chemiluminescence detection kit is used to detect the proteins. This kit is an immune-detection system that uses horseradish peroxidase-conjugated anti-mouse, anti-rabbit, or anti-rat antibodies for the detection of proteins.
Due to its highly sensitive nature, broad range, and more STN ratio, enhanced chemiluminescence is one of the popular detection methods for western blotting applications around the world. This method is also useful in the measurement of a wide variety of biological substances like cells, proteins, RNA, DNA, and other analytes.