All You Need to Know About Multiplex Cytokine Assay

Cytokines are tiny cell-signaling protein molecules that help in cellular communication, immune response, inflammation, and hematopoiesis to takes place. Cytokine Assay kits are used for the detection of these molecules for research and medical purposes.

Studying cell signaling through assay systems is reliable and effective when the ideal detection mechanism is performed. Mouse, human, and rat detection of cytokine essays are available online. You can buy high-quality multiplex cytokine assay at

The main features to look for in a cytokine assay kit are that they are sensitive, have a broad range, and have high-quality antibody pairs. The diversity in cytokine correspond to the inflammation and cancer is wide, and there is a constant need to measure multiple cytokines present in cell, serum, and plasma.

Multiplex technologies in Cytokine assays help in the detection of multiple biomarkers, all at the same time.  The complicated mixture of multiple antibodies, both bead-associated and reporter-conjugated is the limiting factor in the process of extensive multiplexing.

Also, all the antibodies need to be highly specific and sensitive to avoid any cross-reactivity in the sample set given for testing. The different sensitivities of the kits can be explained by the different antibodies used in the assay kits. It even varies according to the variation in incubation times recommended by manufacturers for the samples and beads.

All You Need to Know About Calnexin Antibody

Calnexin Antibody is a high-quality monoclonal which is used for the detection of the protein named Calnexin in mouse, rat, and humans. Calnexin Antibody is available as both the non-conjugated anti-Calnexin antibody and multiple conjugated anti-Calnexin antibodies.

Calnexin and Calregulin are the two calcium linked proteins that are local to the endoplasmic reticulum. Calnexin is local to the membrane and Calregulin to the lumen. You can buy a high-quality calnexin antibody at

Calnexin is a type I membrane protein that communicates with newly synthesized glycoproteins present locally in the endoplasmic reticulum or ER. It helps in assembling the protein and in retaining unassembled proteins inside the ER. Calregulin has both low and high-affinity calcium-linking sites.

Both Calnexin and Calregulin do not contain the calcium-binding “E-F hand” motif which can be found in calmodulins. Both, Calnexin and Calregulin are important for the maturation of glycoproteins present inside the endoplasmic reticulum and can bind many of the same proteins.

So, calnexin is a calcium-bound protein embedded inside the ER membrane that is used to hold the freshly synthesized glycoproteins present inside the ER to ensure their proper folding and quality control. The specifics of Calnexin for a subset of glycoproteins is defined by a lectin site, which links an early oligosaccharide which intermediates on the folding glycoprotein.